Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Pulsed field gel electrophoresis was carried out on lymphocyte DNA obtained from ten patients with the inherited premalignant condition adenomatous polyposis coli and a similar number of control samples to search for large scale molecular rearrangements in the vicinity of the APC locus. DNA from one patient showed a rearranged pattern which was interpreted as evidence for an inversion of approximately 150 kb, changing the transcriptional orientation of the neighbouring MCC gene and bringing it closer to APC. Such an interpretation was supported by fluorescent in situ hybridization data.

Type

Journal article

Journal

Ann Hum Genet

Publication Date

07/1993

Volume

57

Pages

169 - 178

Keywords

Adenomatous Polyposis Coli, Chromosome Inversion, Chromosomes, Artificial, Yeast, DNA, Electrophoresis, Agar Gel, Gene Rearrangement, Genes, APC, Genes, MCC, Humans, In Situ Hybridization, Fluorescence, Lymphocytes, Precancerous Conditions, Restriction Mapping