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We describe a generic, GFP-based pipeline for membrane protein overexpression and purification in Escherichia coli. We exemplify the use of the pipeline by the identification and characterization of E. coli YedZ, a new, membrane-integral flavocytochrome. The approach is scalable and suitable for high-throughput applications. The GFP-based pipeline will facilitate the characterization of the E. coli membrane proteome and serves as an important reference for the characterization of other membrane proteomes.

Original publication

DOI

10.1110/ps.051466205

Type

Journal article

Journal

Protein Sci

Publication Date

08/2005

Volume

14

Pages

2011 - 2017

Keywords

Escherichia coli, Escherichia coli Proteins, Green Fluorescent Proteins, Lactococcus lactis, Luminescent Agents, Membrane Proteins, Recombinant Fusion Proteins