This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.
Journal article
Methods Mol Biol
2019
1899
67 - 83
CD25, CliniMACS, GMP, Leukapheresis collection, Magnetic beads, Antigens, CD19, B-Lymphocytes, CD4 Antigens, CD8-Positive T-Lymphocytes, Cell Separation, Humans, Immunomagnetic Separation, Interleukin-2 Receptor alpha Subunit, Leukapheresis, T-Lymphocytes