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This SOP describes a closed system for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis collections (nMLCs), independent of a clean room in a certified GMP premises, by using CliniMACS format GMP grade reagents (CD25-labeled magnetic beads with/without pre-depletion of CD8+ T cells and CD19+ B cells), a GMP grade-A laminar hood and CliniMACS cell processing system.

Original publication

DOI

10.1007/978-1-4939-8938-6_6

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2019

Volume

1899

Pages

67 - 83

Keywords

CD25, CliniMACS, GMP, Leukapheresis collection, Magnetic beads, Antigens, CD19, B-Lymphocytes, CD4 Antigens, CD8-Positive T-Lymphocytes, Cell Separation, Humans, Immunomagnetic Separation, Interleukin-2 Receptor alpha Subunit, Leukapheresis, T-Lymphocytes