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Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein. Binding of the Fe-S cluster is mediated by an evolutionarily conserved domain at the C terminus of the large subunit. We further show that the Fe-S domain is essential to the unique ability of the eukaryotic primase to start DNA replication.

Original publication

DOI

10.1038/nsmb1288

Type

Journal article

Journal

Nat Struct Mol Biol

Publication Date

09/2007

Volume

14

Pages

875 - 877

Keywords

DNA Primase, Electron Spin Resonance Spectroscopy, Iron-Sulfur Proteins, Protein Binding, RNA