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This study investigated the interaction of human circulating angiogenic cells (CACs) with a degradable polar hydrophobic ionic polyurethane (D-PHI) which has been previously shown to exhibit anti-inflammatory character and favorable interactions with human endothelial cells (ECs). Given the implication of the CACs in microvessel development it was of intrinsic interest to expand our knowledge of D-PHI biocompatibility with this relevant primary cell involved in angiogenesis. The findings will be compared to a well-established benchmark substrate for CACs, fibronectin-coated tissue culture polystyrene (TCPS). Immunoblotting analysis showed that CACs were a heterogeneous population of cells composed mostly of monocytic cells expressing the CD14 marker. Assessment of the cytokine release profile, using ELISA, showed that D-PHI supported a higher concentration of interleukin-10 (IL-10) when compared to the concentration of tumor necrosis factor alpha, which is indicative of an anti-inflammatory phenotype, and was different from the response with TCPS. It was found that the CACs were attached to D-PHI and remained viable and functional (nitric oxide production) during the seven days of culture. However, there did not appear to be any significant proliferation on D-PHI, contrary to the CAC growth on fibronectin-coated TCPS. It was concluded that D-PHI displayed some of the qualities suitable to enable the retention of CACs onto this substrate, as well as maintaining an anti-inflammatory phenotype, characteristics which have been reported to be important for angiogenesis in vivo.

Original publication




Journal article


J Biomater Sci Polym Ed

Publication Date





1159 - 1173


circulating angiogenic cells, degradable, monocytes, polyurethane, vascular graft, Biocompatible Materials, Cell Adhesion, Cell Survival, Cytokines, Fibronectins, Humans, Hydrophobic and Hydrophilic Interactions, In Vitro Techniques, Interleukin-10, Lipopolysaccharide Receptors, Microscopy, Electron, Scanning, Monocytes, Neovascularization, Physiologic, Nitric Oxide, Platelet Endothelial Cell Adhesion Molecule-1, Polyurethanes, Tumor Necrosis Factor-alpha