Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries

Appeltant R., Adeniran BV., Williams SA.

Lay summary To visualise tissues to determine the presence of disease or simply to understand anatomy, it is important to preserve fresh tissue. Fixatives are chemical solutions that preserve tissues to enable microscopic evaluation. However, some fixatives introduce artefact such as shrinkage of cells. Recently, a new fixative, Form-Acetic, was developed that is superior for preserving the structure of ovary tissue and allows investigation of ovary composition. One component of the ovary is hyaluronic acid (HA), which plays a crucial role in normal ovary function and fertility. Importantly, HA is sensitive to different fixative solutions. Therefore, it is meaningful to verify whether Form-Acetic is suitable for detecting HA. In this study, adult mouse ovaries were fixed in Form-Acetic and HA was detected. All HA-containing structures in the ovary were clearly distinguished which proves that the novel fixative allows the detection of HA.

DOI

10.1530/raf-21-0085

Type

Journal article

Journal

Reproduction and Fertility

Publisher

Bioscientifica

Publication Date

30/12/2021

Volume

2

Pages

L10 - L12

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