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The biological formate hydrogenlyase (FHL) complex links a formate dehydrogenase (FDH) to a hydrogenase (H2ase) and produces H2 and CO2 from formate via mixed-acid fermenta-tion in Escherichia coli. Here, we describe an electrochemical and a colloidal semi-artificial FHL system that consists of an FDH and a H2ase immobilized on conductive indium tin oxide (ITO) as an electron relay. These in vitro systems benefit from the efficient wiring of a highly active enzyme pair and allow for the reversible conversion of formate to H2 and CO2 under ambient temperature and pressure. Such hybrid systems pro-vide a template for the design of analogous synthetic catalysts and surpass the FHL complex in vivo by storing and releasing H2 on demand by interconverting CO2/H2 and formate with minimal bias in either direction.

Original publication

DOI

10.1021/jacs.9b09575

Type

Journal article

Journal

Journal of the American Chemical Society

Publication Date

22/10/2019